Materi paling akhir biologi SMA yang aplikasi diharapkan bisa menambah semangat setidaknya mengerjakan soal bioteknologi atau untuk keinginan mau melanjutkan kemana setelah jagoan biologi OK
1. Bacillus thuringiensis is used to control
A Nematodes
B Fungal pathogens
C Bacterial pathogens
D Insect pests
ANSWER : D
2. The genetically-modified (GM) brinjal in India has been developed for
A Enhancing shelf life
B Insect-resistance
C Drought-resistance
D Enhancing mineral content
ANSWER : B
3. The protein products of the following Bt toxin genes cry IA c and cry II Ab are responsible for controlling
A Bollworm
B Roundworm
C Moth
D Fruit fly
ANSWER : A
4. Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in
A Monoclonal antibody production
B DNA fingerprinting
C Recombinant DNA technology
D Stemcell culture
ANSWER : B
5. Silencing of mRNA has been used in producing transgenic plants resistant to
A Bollworms
B Bacterial blights
C White rusts
D Nematodes
ANSWER : D
6. Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from
A Bacteriophages
B Bacterial cells
C Plasmids
D All prokaryotic cells
ANSWER : B
7 PCR proceeds in three distinct steps governed by temperature, they are in order of
A Annealing, Synthesis, Denaturation
B Synthesis, Annealing, Denaturation
C Denaturation, Annealing, Synthesis
D Denaturation, Synthesis, Annealing
ANSWER : C
8. Which of the following is correctly matched?
A Ligase - Molecular scissors
B Thermus aquaticus - Bt-gene
C Agrobacterium tumefaciens - Tumour
D Hind II - Plasmid vector
ANSWER : C
9 Sterilization of tissue culture medium is done by
A mixing the medium with antifungal agents
B filtering the medium through fine sieve
C autoclaving of medium at 120° for 15 min
D keeping the medium at –20°C
ANSWER : C
10. Biolistics (gene-gun) is suitable for
A Constructing recombinant DNA by joining with vectors
B Transformation of plant cells
C Disarming pathogen vectors
D DNA finger printing
ANSWER : B
11. An improved variety of transgenic basmati rice
A Gives high yield and is rich in vitamin A
B Does not require chemical fertilizers and growth hormones
C Give high yield but has no characteristic aroma
D Is completely resistant to all insect pests and diseases of paddy
ANSWER : C
12. In cloning of cattle a fertilized egg is taken out of the mother's womb and
A. in the eight cell stage the individual cells are separated under electrical field for further development in culture media
B. the egg is divided into 4 pairs of cells which are implanted into the womb of other cows
C. in the eight cell stage, cells are separated and cultured until small embryos are formed which are implanted into the womb of other cows.
D. from this up to eight identical twins can be produced
ANSWER : C
13. Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
A 5′ CACGTA 3′; 3′ CTCAGT 5′
B 5′ CGTTCG 3′; 3′ ATGGTA 5′
C 5′ GATATG 3′; 3′ CTACTA 5′
D 5′ GAATTC 3′; 3′ CTTAAG 5′
ANSWER : D
14. Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason: Both bacteria and yeast multiply very fast to form huge population, which express the desired gene.
A Both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.
B Both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion.
C Assertion is true statement but Reason is false.
D Both Assertion and Reason are false statements.
ANSWER : D
15. Compare the statements A and B.
Statement A: Synthesis of DNA takes place in the S-phase of interphase.
Statement B: Every chromosome, during metaphase, has two chromatids.
Choose the correct description :
A Statement A is correct and B is wrong.
B Statement A is wrong and B is correct.
C Both the statements A and B are correct and A is not the reason for B.
D Both the statements A and B are correct and A is the reason for B.
ANSWER : D
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1. The first clinical gene therapy was given for treating?
A Rheumatoid arthritis
B Chicken pox
C Diabetes mellitus
D Adenosine deaminase deficiency
ANSWER : D
2. Emasculation is related to
A Clonal selection
B Mass selection
C Pure line
D Hybridization
ANSWER : D
3. Which of the following terms is used to describe the component isolated from a plant, for in vitro culturing in the specific medium?
A Synthetic seeds
B Embryoid
C Callus
D Explant
ANSWER : D
4. Nucleic acid segment tagged with a radioactive molecule is called
A plasmid
B probe
C clone
D vector
ANSWER : B
5. Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?
A Exonuclease
B Protease
C Lipase
D Endonuclease
ANSWER : D
6. The enzyme employed for amplification of DNA during PCR is commercially obtained from
A Trichoderma reesei
B Bacillus licheniformis
C Streptococcus pyogenes
D Thermus aquaticus
ANSWER : D
7. A regulatory body working under MoEF for the release of transgenic crops is
A NBPGR
B GEAC
C NSC
D NIPGR
ANSWER : B
8. Cry1 endotoxins obtained from Bacillus Thuringiensis are effective against:
A Flies
B Mosquitoes
C Boll worms
D Nematodes
ANSWER : C
9. In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector
Statements
(A) bacterium
(B) Plasmid
(C) Plasmodium
(D) Bacteriophage
Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
A (A), (B) and (D) only
B (A) only
C (A) and (C) only
D (B) and (D) only
ANSWER : D
10. Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules
(C) Downstream processing is one of the steps of R-DNA technology
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host
Which are the two statements having mistakes?
A Statements (A) and (C)
B Statements (A) and (B)
C Statements (B) and (C)
D Statements (C) and (D)
ANSWER : B
11. Cry II Ab and Cry I Ab produce toxins that control
A Tobacco budworms and nematodes respectively
B Corn borer and cotton bollworms respectively
C Cotton bollworms and corn borer respectively
D Nematodes and tobacco budworms respectively
ANSWER : C
12. Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein
A Binds with epithelial cells of midgut of the insect pest ultimately killing it
B Does not kill the carrier bacterium which is itself resistant to this toxin
C Is activated by acid pH of the foregut of the insect pest
D Is coded by several genes including the gene cry
ANSWER : A
13. What is true about Bt toxin?
A Bt protein exists as active toxin in the Bacillus
B The inactive protoxin gets converted into active form in the insect gut
C The concerned Bacillus has antitoxins
D The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication
ANSWER : B
14. Assertion : In plant tissue culture, somatic embryos can be induced from any plant cell. Reason : Any viable plant cell can differentiate in to somatic embryos.
A Both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.
B Both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion.
C Assertion is true statement but Reason is false.
D Both Assertion and Reason are false statements.
ANSWER : A
15. Which one of the following is a correct statement?
A The anticoagulant hirudin is being produced from transgenic Brassica napus seeds
B Somatic hybridization involves fusion of two complete plant cells carrying desired genes
C "Bt" in "Bt-cotton" indicates that it is a genetically modified organism produced through biotechnology
D "Flavr Savr" variety of tomato has enhanced the production of ethylene which improves its taste
ANSWER : A
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